| 2. Round | 05/08/2000 | Lüneburger SK | - | 1. FC Union Berlin | 1:2 | ||||
| 4. Round | 20/08/2000 | 1. FC Union Berlin | - | SG Wattenscheid 09 | 3:0 | ||||
| 10. Round | 30/09/2000 | 1. FC Union Berlin | - | SV Babelsberg 03 | 3:0 | ||||
| 11. Round | 08/10/2000 | Preußen Münster | - | 1. FC Union Berlin | 1:1 |  |
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| 12. Round | 14/10/2000 | 1. FC Union Berlin | - | Fortuna Düsseldorf | 2:1 | ||||
| 13. Round | 22/10/2000 | Werder Bremen II | - | 1. FC Union Berlin | 0:1 | ||||
| 14. Round | 28/10/2000 | 1. FC Union Berlin | - | Borussia Dortmund II | 1:1 | ||||
| 15. Round | 04/11/2000 | SV Wilhelmshaven | - | 1. FC Union Berlin | 2:2 | ||||
| 16. Round | 11/11/2000 | 1. FC Union Berlin | - | Dresdner SC | 2:0 | ||||
| 17. Round | 17/11/2000 | Rot-Weiss Essen | - | 1. FC Union Berlin | 0:0 | ||||
| 18. Round | 25/11/2000 | 1. FC Union Berlin | - | SC Verl | 3:0 | ||||
| 19. Round | 02/12/2000 | KFC Uerdingen 05 | - | 1. FC Union Berlin | 1:1 | ||||
| 21. Round | 05/12/2000 | 1. FC Union Berlin | - | Lüneburger SK | 1:2 | |
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| 38. Round | 09/06/2001 | 1. FC Union Berlin | - | KFC Uerdingen 05 | 4:0 |
Germany