| 20. Round | 28/02/2010 | Stuttgarter Kickers | - | SpVgg Weiden | 2:0 | ||||
| 21. Round | 05/03/2010 | TSV 1860 München II | - | Stuttgarter Kickers | 2:2 | ||||
| 23. Round | 19/03/2010 | SSV Ulm 1846 | - | Stuttgarter Kickers | 0:1 | ||||
| 28. Round | 23/04/2010 | Stuttgarter Kickers | - | Eintracht Frankfurt II | 2:2 |  |
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| 18. Round | 27/04/2010 | Stuttgarter Kickers | - | SC Freiburg II | 2:1 | ||||
| 29. Round | 02/05/2010 | 1. FC Eintracht Bamberg | - | Stuttgarter Kickers | 1:1 | |
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| 30. Round | 07/05/2010 | Stuttgarter Kickers | - | Bayern Alzenau | 3:0 | ||||
| 31. Round | 12/05/2010 | SSV Reutlingen | - | Stuttgarter Kickers | 1:3 |  |
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| 32. Round | 15/05/2010 | Stuttgarter Kickers | - | Hessen Kassel | 3:3 | ||||
| 33. Round | 22/05/2010 | VfR Aalen | - | Stuttgarter Kickers | 2:0 | ||||
| 34. Round | 29/05/2010 | Stuttgarter Kickers | - | 1. FC Nürnberg II | 0:4 | |
Germany