| 27. Round | 14/02/2014 | VVV-Venlo | - | Jong FC Twente | 3:0 | ||||
| 28. Round | 21/02/2014 | Fortuna Sittard | - | Jong FC Twente | 4:1 | ||||
| 29. Round | 28/02/2014 | Jong FC Twente | - | Willem II | 1:2 | ||||
| 30. Round | 09/03/2014 | Sparta Rotterdam | - | Jong FC Twente | 0:1 | ||||
| 32. Round | 21/03/2014 | FC Dordrecht | - | Jong FC Twente | 3:1 | ||||
| 33. Round | 29/03/2014 | FC Emmen | - | Jong FC Twente | 2:0 | ||||
| 36. Round | 18/04/2014 | Helmond Sport | - | Jong FC Twente | 5:1 | ||||
| 37. Round | 21/04/2014 | Jong FC Twente | - | SBV Excelsior | 1:2 |
Germany