| 7. Round | 11/09/2009 | Hertha BSC II | - | Chemnitzer FC | 2:1 | ||||
| 10. Round | 02/10/2009 | TeBe Berlin | - | Hertha BSC II | 0:2 | ||||
| 12. Round | 23/10/2009 | 1. FC Magdeburg | - | Hertha BSC II | 3:0 | ||||
| 13. Round | 31/10/2009 | Hertha BSC II | - | Hamburger SV II | 0:1 | ||||
| 14. Round | 06/11/2009 | Türkiyemspor Berlin | - | Hertha BSC II | 3:1 |  |
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| 17. Round | 05/12/2009 | Hertha BSC II | - | SV Wilhelmshaven | 1:3 | ||||
| 19. Round | 20/12/2009 | Hannover 96 II | - | Hertha BSC II | 3:3 | ||||
| 23. Round | 06/03/2010 | VfB Lübeck | - | Hertha BSC II | 1:1 |  |
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| 24. Round | 14/03/2010 | Chemnitzer FC | - | Hertha BSC II | 2:2 | ||||
| 25. Round | 20/03/2010 | Hertha BSC II | - | VfL Wolfsburg II | 1:3 | ||||
| 26. Round | 28/03/2010 | FC Oberneuland | - | Hertha BSC II | 1:1 | ||||
| 27. Round | 02/04/2010 | Hertha BSC II | - | TeBe Berlin | 1:1 | ||||
| 28. Round | 11/04/2010 | ZFC Meuselwitz | - | Hertha BSC II | 2:1 |
Germany