| 1. Round | 05/09/2020 | 1899 Hoffenheim II | - | 1. FSV Mainz 05 II | 1:2 | ||||
| 15. Round | 16/01/2021 | FSV Frankfurt | - | 1. FSV Mainz 05 II | 2:1 |  |
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| 16. Round | 19/01/2021 | 1. FSV Mainz 05 II | - | FC-Astoria Walldorf | 0:2 | ||||
| 33. Round | 20/04/2021 | FC 08 Homburg | - | 1. FSV Mainz 05 II | 2:2 | ||||
| 38. Round | 16/05/2021 | 1. FSV Mainz 05 II | - | VfR Aalen | 3:1 | |
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| 39. Round | 22/05/2021 | FK Pirmasens | - | 1. FSV Mainz 05 II | 2:0 | ||||
| 40. Round | 29/05/2021 | Bahlinger SC | - | 1. FSV Mainz 05 II | 2:1 | ||||
| 41. Round | 05/06/2021 | 1. FSV Mainz 05 II | - | SG Sonnenhof Großaspach | 0:1 | ||||
| 42. Round | 11/06/2021 | Kickers Offenbach | - | 1. FSV Mainz 05 II | 1:0 | |
Germany