| 3. Round | 22/08/2009 | SV 07 Elversberg | - | Fortuna Düsseldorf II | 0:3 | ||||
| 4. Round | 28/08/2009 | Fortuna Düsseldorf II | - | Eintracht Trier | 2:1 | ||||
| 6. Round | 04/09/2009 | Fortuna Düsseldorf II | - | Preußen Münster | 1:2 | ||||
| 9. Round | 26/09/2009 | Waldhof Mannheim | - | Fortuna Düsseldorf II | 4:0 | ||||
| 11. Round | 18/10/2009 | 1. FSV Mainz 05 II | - | Fortuna Düsseldorf II | 2:0 | ||||
| 18. Round | 12/12/2009 | Fortuna Düsseldorf II | - | 1. FC Köln II | 1:1 | ||||
| 25. Round | 19/03/2010 | Bor. Mönchengladbach II | - | Fortuna Düsseldorf II | 0:3 | ||||
| 31. Round | 02/05/2010 | 1. FC Saarbrücken | - | Fortuna Düsseldorf II | 2:1 | ||||
| 32. Round | 08/05/2010 | Fortuna Düsseldorf II | - | SC Verl | 0:4 |
Germany