| 1. Round | 05/09/2020 | VfB Stuttgart II | - | Hessen Kassel | 0:2 | ||||
| 2. Round | 11/09/2020 | FC 08 Homburg | - | VfB Stuttgart II | 3:0 | ||||
| 4. Round | 14/10/2020 | Rot-Weiß Koblenz | - | VfB Stuttgart II | 1:0 | ||||
| 9. Round | 17/10/2020 | VfB Stuttgart II | - | Bahlinger SC | 6:2 | ||||
| 24. Round | 20/02/2021 | SV 07 Elversberg | - | VfB Stuttgart II | 4:0 | ||||
| 32. Round | 17/04/2021 | Kickers Offenbach | - | VfB Stuttgart II | 1:1 | ||||
| 33. Round | 20/04/2021 | VfB Stuttgart II | - | 1899 Hoffenheim II | 2:1 |  |
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| 34. Round | 24/04/2021 | TSG Balingen | - | VfB Stuttgart II | 1:3 | ||||
| 35. Round | 30/04/2021 | TSV Steinbach Haiger | - | VfB Stuttgart II | 1:0 | ||||
| 36. Round | 04/05/2021 | SC Freiburg II | - | VfB Stuttgart II | 3:1 | |
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| 37. Round | 08/05/2021 | VfB Stuttgart II | - | Bayern Alzenau | 3:2 | ||||
| 38. Round | 15/05/2021 | SSV Ulm 1846 | - | VfB Stuttgart II | 2:0 | ||||
| 40. Round | 29/05/2021 | VfB Stuttgart II | - | FK Pirmasens | 3:1 | ||||
| 41. Round | 05/06/2021 | TSV Schott Mainz | - | VfB Stuttgart II | 2:4 | |
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| 42. Round | 12/06/2021 | VfB Stuttgart II | - | Eintracht Stadtallendorf | 7:1 |
Germany