| 18. Round | 17/03/2010 | ZFC Meuselwitz | - | Hamburger SV II | 2:1 | ||||
| 22. Round | 31/03/2010 | Hamburger SV II | - | Hansa Rostock II | 3:0 |  |
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| 27. Round | 03/04/2010 | Hallescher FC | - | Hamburger SV II | 0:1 | ||||
| 28. Round | 10/04/2010 | Hamburger SV II | - | Goslarer SC | 2:1 | ||||
| 21. Round | 14/04/2010 | Türkiyemspor Berlin | - | Hamburger SV II | 0:1 | ||||
| 29. Round | 17/04/2010 | FC St. Pauli II | - | Hamburger SV II | 0:3 | |
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| 30. Round | 25/04/2010 | Hamburger SV II | - | Hertha BSC II | 1:2 |
Germany