| 15. Round | 20/02/2010 | Karlsruher SC [Youth B] | - | Bayern München [Youth B] | 1:3 | ||||
| 16. Round | 28/02/2010 | Wacker Burghausen [Youth B] | - | Karlsruher SC [Youth B] | 3:3 | ||||
| 18. Round | 20/03/2010 | Karlsruher SC [Youth B] | - | TSV 1860 München [Youth B] | 0:1 | ||||
| 19. Round | 28/03/2010 | TuS Ergenzingen [Youth B] | - | Karlsruher SC [Youth B] | 0:2 | ||||
| 22. Round | 25/04/2010 | Karlsruher SC [Youth B] | - | FK Pirmasens [Youth B] | 4:1 | ||||
| 23. Round | 08/05/2010 | SpVgg Greuther Fürth [Youth B] | - | Karlsruher SC [Youth B] | 1:0 | ||||
| 24. Round | 16/05/2010 | Karlsruher SC [Youth B] | - | SC Freiburg [Youth B] | 3:4 | ||||
| 25. Round | 06/06/2010 | 1. FC Nürnberg [Youth B] | - | Karlsruher SC [Youth B] | 1:1 |
Germany