| 5. Round | 02/09/2009 | VFC Plauen | - | 1. FC Magdeburg | 1:4 | ||||
| 19. Round | 24/03/2010 | Hamburger SV II | - | 1. FC Magdeburg | 1:1 |  |
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| 26. Round | 28/03/2010 | 1. FC Magdeburg | - | Hallescher FC | 0:1 | ||||
| 27. Round | 04/04/2010 | Goslarer SC | - | 1. FC Magdeburg | 1:1 | ||||
| 28. Round | 11/04/2010 | 1. FC Magdeburg | - | FC St. Pauli II | 1:0 | ||||
| 29. Round | 18/04/2010 | Hertha BSC II | - | 1. FC Magdeburg | 3:0 | ||||
| 30. Round | 25/04/2010 | 1. FC Magdeburg | - | Chemnitzer FC | 1:0 | ||||
| 32. Round | 09/05/2010 | 1. FC Magdeburg | - | FC Oberneuland | 5:0 | ||||
| 34. Round | 29/05/2010 | 1. FC Magdeburg | - | ZFC Meuselwitz | 1:1 |
Germany