| 1. Round | 07/08/2009 | SV Wehen Wiesbaden II | - | 1. FC Nürnberg II | 1:2 | ||||
| 2. Round | 14/08/2009 | Stuttgarter Kickers | - | SV Wehen Wiesbaden II | 2:0 | ||||
| 10. Round | 03/10/2009 | SV Wehen Wiesbaden II | - | SC Pfullendorf | 0:0 |  |
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| 11. Round | 17/10/2009 | Karlsruher SC II | - | SV Wehen Wiesbaden II | 3:0 | |
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| 12. Round | 24/10/2009 | SV Wehen Wiesbaden II | - | Eintracht Frankfurt II | 1:5 | ||||
| 21. Round | 27/04/2010 | SpVgg Weiden | - | SV Wehen Wiesbaden II | 2:1 |  |
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| 31. Round | 12/05/2010 | Bayern Alzenau | - | SV Wehen Wiesbaden II | 1:0 | ||||
| 32. Round | 15/05/2010 | SV Wehen Wiesbaden II | - | SSV Reutlingen | 0:3 | ||||
| 33. Round | 22/05/2010 | Hessen Kassel | - | SV Wehen Wiesbaden II | 1:0 | ||||
| 34. Round | 29/05/2010 | SV Wehen Wiesbaden II | - | VfR Aalen | 0:2 |
Germany