| 13. Round | 05/12/2009 | Hannover 96 [Youth B] | - | 1. FC Union Berlin [Youth B] | 1:2 |  |
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| 16. Round | 28/02/2010 | Werder Bremen [Youth B] | - | 1. FC Union Berlin [Youth B] | 4:1 | ||||
| 18. Round | 20/03/2010 | Hansa Rostock [Youth B] | - | 1. FC Union Berlin [Youth B] | 1:1 | |
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| 19. Round | 28/03/2010 | 1. FC Union Berlin [Youth B] | - | FC Carl Zeiss Jena [Youth B] | 2:0 | ||||
| 21. Round | 18/04/2010 | Holstein Kiel [Youth B] | - | 1. FC Union Berlin [Youth B] | 4:1 | ||||
| 22. Round | 24/04/2010 | 1. FC Union Berlin [Youth B] | - | Hamburger SV [Youth B] | 1:0 | ||||
| 14. Round | 01/05/2010 | VfL Wolfsburg [Youth B] | - | 1. FC Union Berlin [Youth B] | 0:1 | |
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| 23. Round | 08/05/2010 | VfL Osnabrück [Youth B] | - | 1. FC Union Berlin [Youth B] | 6:0 | ||||
| 15. Round | 29/05/2010 | 1. FC Union Berlin [Youth B] | - | Energie Cottbus [Youth B] | 1:2 | ||||
| 25. Round | 06/06/2010 | TeBe Berlin [Youth B] | - | 1. FC Union Berlin [Youth B] | 5:2 |
Germany