| 1. Round | 25/07/2009 | Eintracht Braunschweig | - | VfL Osnabrück | 1:0 | ||||
| 2. Round | 28/07/2009 | VfL Osnabrück | - | Werder Bremen II | 1:0 | ||||
| 3. Round | 08/08/2009 | Kickers Offenbach | - | VfL Osnabrück | 2:0 | ||||
| 4. Round | 15/08/2009 | VfL Osnabrück | - | FC Ingolstadt 04 | 5:2 | ||||
| 5. Round | 22/08/2009 | SpVgg Unterhaching | - | VfL Osnabrück | 1:0 | ||||
| 6. Round | 29/08/2009 | VfL Osnabrück | - | FC Carl Zeiss Jena | 2:0 |  |
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| 18. Round | 28/11/2009 | Holstein Kiel | - | VfL Osnabrück | 1:1 | ||||
| 22. Round | 31/01/2010 | VfL Osnabrück | - | Kickers Offenbach | 1:0 | |
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| 23. Round | 06/02/2010 | FC Ingolstadt 04 | - | VfL Osnabrück | 0:0 | ||||
| 26. Round | 27/02/2010 | VfL Osnabrück | - | Dynamo Dresden | 1:1 |
Germany