| 1. Round | 08/08/2009 | 1. FC Köln II | - | Fortuna Düsseldorf II | 2:2 | ||||
| 2. Round | 14/08/2009 | Fortuna Düsseldorf II | - | Wormatia Worms | 1:1 | ||||
| 3. Round | 22/08/2009 | SV 07 Elversberg | - | Fortuna Düsseldorf II | 0:3 | ||||
| 7. Round | 11/09/2009 | Sportfreunde Lotte | - | Fortuna Düsseldorf II | 1:0 | ||||
| 8. Round | 19/09/2009 | Fortuna Düsseldorf II | - | Bor. Mönchengladbach II | 2:1 | ||||
| 9. Round | 26/09/2009 | Waldhof Mannheim | - | Fortuna Düsseldorf II | 4:0 | ||||
| 10. Round | 02/10/2009 | Fortuna Düsseldorf II | - | VfL Bochum II | 1:0 |  |
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| 15. Round | 21/11/2009 | SC Verl | - | Fortuna Düsseldorf II | 1:2 | ||||
| 22. Round | 01/03/2010 | Fortuna Düsseldorf II | - | Rot-Weiss Essen | 0:2 | ||||
| 24. Round | 13/03/2010 | Fortuna Düsseldorf II | - | Sportfreunde Lotte | 0:2 | |
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| 26. Round | 29/03/2010 | Fortuna Düsseldorf II | - | Waldhof Mannheim | 0:0 | ||||
| 27. Round | 03/04/2010 | VfL Bochum II | - | Fortuna Düsseldorf II | 2:1 | ||||
| 29. Round | 18/04/2010 | 1. FC Kaiserslautern II | - | Fortuna Düsseldorf II | 2:2 | ||||
| 30. Round | 24/04/2010 | Fortuna Düsseldorf II | - | FC Schalke 04 II | 0:4 |
Germany