| 8. Round | 19/09/2009 | Fortuna Düsseldorf II | - | Bor. Mönchengladbach II | 2:1 |  |
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| 10. Round | 02/10/2009 | Fortuna Düsseldorf II | - | VfL Bochum II | 1:0 | ||||
| 13. Round | 31/10/2009 | FC Schalke 04 II | - | Fortuna Düsseldorf II | 3:0 | |
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| 21. Round | 20/02/2010 | Eintracht Trier | - | Fortuna Düsseldorf II | 0:3 | |
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| 19. Round | 23/02/2010 | Wormatia Worms | - | Fortuna Düsseldorf II | 2:1 | |
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| 22. Round | 01/03/2010 | Fortuna Düsseldorf II | - | Rot-Weiss Essen | 0:2 | |
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| 24. Round | 13/03/2010 | Fortuna Düsseldorf II | - | Sportfreunde Lotte | 0:2 | |
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| 25. Round | 19/03/2010 | Bor. Mönchengladbach II | - | Fortuna Düsseldorf II | 0:3 | ||||
| 26. Round | 29/03/2010 | Fortuna Düsseldorf II | - | Waldhof Mannheim | 0:0 | ||||
| 27. Round | 03/04/2010 | VfL Bochum II | - | Fortuna Düsseldorf II | 2:1 | ||||
| 29. Round | 18/04/2010 | 1. FC Kaiserslautern II | - | Fortuna Düsseldorf II | 2:2 | |
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| 30. Round | 24/04/2010 | Fortuna Düsseldorf II | - | FC Schalke 04 II | 0:4 | |
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| 31. Round | 02/05/2010 | 1. FC Saarbrücken | - | Fortuna Düsseldorf II | 2:1 | |
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| 32. Round | 08/05/2010 | Fortuna Düsseldorf II | - | SC Verl | 0:4 | |
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| 34. Round | 29/05/2010 | Bonner SC | - | Fortuna Düsseldorf II | 1:2 |
Germany