| 24. Round | 14/02/2010 | VfL Osnabrück | - | SpVgg Unterhaching | 1:0 | ||||
| 25. Round | 21/02/2010 | SpVgg Unterhaching | - | Borussia Dortmund II | 1:1 |  |
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| 22. Round | 09/03/2010 | Erzgebirge Aue | - | SpVgg Unterhaching | 2:0 | ||||
| 28. Round | 13/03/2010 | Eintracht Braunschweig | - | SpVgg Unterhaching | 1:0 | |
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| 29. Round | 21/03/2010 | SpVgg Unterhaching | - | Werder Bremen II | 0:1 | |
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| 26. Round | 24/03/2010 | Holstein Kiel | - | SpVgg Unterhaching | 2:2 |  |
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| 30. Round | 27/03/2010 | Kickers Offenbach | - | SpVgg Unterhaching | 1:3 | ||||
| 32. Round | 03/04/2010 | SpVgg Unterhaching | - | Jahn Regensburg | 2:0 | ||||
| 33. Round | 10/04/2010 | FC Carl Zeiss Jena | - | SpVgg Unterhaching | 1:1 | ||||
| 34. Round | 13/04/2010 | SpVgg Unterhaching | - | Dynamo Dresden | 0:0 | ||||
| 38. Round | 08/05/2010 | SpVgg Unterhaching | - | SV Wehen Wiesbaden | 4:3 |
Germany