| 28. Round | 26/02/2021 | Fortuna Düsseldorf II | - | Rot-Weiss Essen | 3:0 | ||||
| 29. Round | 07/03/2021 | Rot-Weiss Essen | - | Fortuna Köln | 2:0 | ||||
| 30. Round | 13/03/2021 | Preußen Münster | - | Rot-Weiss Essen | 1:0 | ||||
| 25. Round | 17/03/2021 | Rot-Weiss Essen | - | Borussia Dortmund II | 1:1 | ||||
| 31. Round | 20/03/2021 | Rot-Weiss Essen | - | Bor. Mönchengladbach II | 4:0 | ||||
| 26. Round | 24/03/2021 | Rot Weiss Ahlen | - | Rot-Weiss Essen | 2:1 | ||||
| 32. Round | 27/03/2021 | Rot-Weiß Oberhausen | - | Rot-Weiss Essen | 1:1 | ||||
| 33. Round | 03/04/2021 | Rot-Weiss Essen | - | FC Schalke 04 II | 5:0 |  |
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| 34. Round | 07/04/2021 | SV Rödinghausen | - | Rot-Weiss Essen | 1:1 | ||||
| 35. Round | 10/04/2021 | Rot-Weiss Essen | - | SV Bergisch Gladbach 09 | 4:0 | ||||
| 36. Round | 17/04/2021 | SV 19 Straelen | - | Rot-Weiss Essen | 0:2 | |
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| 37. Round | 24/04/2021 | Rot-Weiss Essen | - | VfB Homberg | 4:1 | ||||
| 27. Round | 02/05/2021 | Rot-Weiss Essen | - | SV Lippstadt 08 | 5:0 | ||||
| 38. Round | 08/05/2021 | Wuppertaler SV | - | Rot-Weiss Essen | 1:2 | |
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| 39. Round | 15/05/2021 | Rot-Weiss Essen | - | Alemannia Aachen | 2:1 | ||||
| 41. Round | 26/05/2021 | Rot-Weiss Essen | - | Sportfreunde Lotte | 5:2 |
Germany