| 19. Round | 26/01/2025 | Türkspor Dortmund | - | MSV Duisburg | annull. |  |
|||
| 20. Round | 31/01/2025 | MSV Duisburg | - | Rot-Weiß Oberhausen | 0:2 | ||||
| 21. Round | 08/02/2025 | Eintracht Hohkeppel | - | MSV Duisburg | 2:2 | ||||
| 22. Round | 14/02/2025 | MSV Duisburg | - | SC Paderborn 07 II | 0:0 | ||||
| 23. Round | 23/02/2025 | Fortuna Düsseldorf II | - | MSV Duisburg | 0:1 | ||||
| 24. Round | 01/03/2025 | MSV Duisburg | - | Fortuna Köln | 2:0 | ||||
| 25. Round | 07/03/2025 | SC Wiedenbrück | - | MSV Duisburg | 0:2 | |
|||
| 26. Round | 14/03/2025 | MSV Duisburg | - | FC Schalke 04 II | 4:0 | ||||
| 27. Round | 29/03/2025 | SV Rödinghausen | - | MSV Duisburg | 0:1 | |
|||
| 28. Round | 04/04/2025 | MSV Duisburg | - | 1. FC Bocholt | 1:1 |  |
|||
| 29. Round | 12/04/2025 | 1. FC Düren | - | MSV Duisburg | 0:6 | |
|||
| 30. Round | 20/04/2025 | MSV Duisburg | - | 1. FC Köln II | 3:1 | ||||
| 31. Round | 25/04/2025 | Bor. Mönchengladbach II | - | MSV Duisburg | 0:1 | ||||
| 32. Round | 03/05/2025 | MSV Duisburg | - | Wuppertaler SV | 3:0 | ||||
| 34. Round | 17/05/2025 | Sportfreunde Lotte | - | MSV Duisburg | 0:4 | |
Germany