| 1. Round | 31/07/2016 | SC Wiedenbrück | - | Rot-Weiss Essen | 0:2 | ||||
| 2. Round | 05/08/2016 | Rot-Weiss Essen | - | Bonner SC | 2:1 | ||||
| 4. Round | 13/08/2016 | Rot-Weiss Essen | - | Viktoria Köln | 0:4 | ||||
| 8. Round | 10/09/2016 | Rot Weiss Ahlen | - | Rot-Weiss Essen | 0:3 |  |
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| 9. Round | 16/09/2016 | Rot-Weiss Essen | - | SC Verl | 2:0 | ||||
| 11. Round | 01/10/2016 | Rot-Weiss Essen | - | Borussia Dortmund II | 1:1 | ||||
| 14. Round | 21/10/2016 | Sportfreunde Siegen | - | Rot-Weiss Essen | 1:5 | ||||
| 16. Round | 06/11/2016 | Rot-Weiß Oberhausen | - | Rot-Weiss Essen | 2:2 | ||||
| 19. Round | 04/12/2016 | Bonner SC | - | Rot-Weiss Essen | 1:2 | ||||
| 20. Round | 11/12/2016 | Rot-Weiss Essen | - | Wuppertaler SV | 1:0 | ||||
| 5. Round | 16/12/2016 | Fortuna Düsseldorf II | - | Rot-Weiss Essen | 2:1 | ||||
| 26. Round | 24/03/2017 | SC Verl | - | Rot-Weiss Essen | 0:0 | ||||
| 27. Round | 31/03/2017 | Rot-Weiss Essen | - | Alemannia Aachen | 1:2 | ||||
| 28. Round | 08/04/2017 | Borussia Dortmund II | - | Rot-Weiss Essen | 1:1 | ||||
| 29. Round | 13/04/2017 | Rot-Weiss Essen | - | FC Schalke 04 II | 1:0 | ||||
| 33. Round | 13/05/2017 | Rot-Weiss Essen | - | Rot-Weiß Oberhausen | 3:2 | ||||
| 34. Round | 20/05/2017 | TSG Sprockhövel | - | Rot-Weiss Essen | 1:1 |  |
Germany