| 1. Round | 05/08/2023 | Hessen Kassel | - | 1. FSV Mainz 05 II | 2:0 | ||||
| 2. Round | 15/08/2023 | 1. FSV Mainz 05 II | - | TSG Balingen | 1:1 | ||||
| 3. Round | 20/08/2023 | 1. FSV Mainz 05 II | - | TSV Steinbach Haiger | 3:0 | ||||
| 4. Round | 26/08/2023 | Stuttgarter Kickers | - | 1. FSV Mainz 05 II | 4:1 | ||||
| 5. Round | 29/08/2023 | 1. FSV Mainz 05 II | - | VfR Aalen | 1:1 | ||||
| 6. Round | 03/09/2023 | Eintracht Frankfurt II | - | 1. FSV Mainz 05 II | 6:1 | ||||
| 7. Round | 19/09/2023 | 1. FSV Mainz 05 II | - | 1899 Hoffenheim II | 1:0 |  |
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| 9. Round | 23/09/2023 | 1. FSV Mainz 05 II | - | TSV Schott Mainz | 3:0 | ||||
| 10. Round | 30/09/2023 | Bahlinger SC | - | 1. FSV Mainz 05 II | 2:1 | ||||
| 11. Round | 03/10/2023 | 1. FSV Mainz 05 II | - | FSV Frankfurt | 2:0 |  |
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| 12. Round | 08/10/2023 | VfB Stuttgart II | - | 1. FSV Mainz 05 II | 4:0 | ||||
| 14. Round | 21/10/2023 | SGV Freiberg | - | 1. FSV Mainz 05 II | 1:2 | |
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| 15. Round | 28/10/2023 | 1. FSV Mainz 05 II | - | FC 08 Homburg | 2:4 | |
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| 16. Round | 04/11/2023 | Kickers Offenbach | - | 1. FSV Mainz 05 II | 1:3 | ||||
| 17. Round | 11/11/2023 | 1. FSV Mainz 05 II | - | TuS Koblenz | 2:1 |
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