| 24. Round | 13/02/2010 | SV Wehen Wiesbaden | - | VfB Stuttgart II | 1:4 | ||||
| 23. Round | 23/02/2010 | Jahn Regensburg | - | VfB Stuttgart II | 0:0 | ||||
| 26. Round | 27/02/2010 | Bayern München II | - | VfB Stuttgart II | 2:0 | ||||
| 28. Round | 16/03/2010 | Rot-Weiß Erfurt | - | VfB Stuttgart II | 0:1 | ||||
| 29. Round | 19/03/2010 | VfB Stuttgart II | - | VfL Osnabrück | 0:1 |  |
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| 25. Round | 23/03/2010 | VfB Stuttgart II | - | SV Sandhausen | 3:0 | ||||
| 30. Round | 27/03/2010 | Borussia Dortmund II | - | VfB Stuttgart II | 1:2 |  |
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| 31. Round | 31/03/2010 | VfB Stuttgart II | - | Holstein Kiel | 1:0 | ||||
| 32. Round | 03/04/2010 | Wacker Burghausen | - | VfB Stuttgart II | 3:0 | ||||
| 27. Round | 07/04/2010 | VfB Stuttgart II | - | Erzgebirge Aue | 1:2 | ||||
| 33. Round | 10/04/2010 | VfB Stuttgart II | - | Eintracht Braunschweig | 1:2 | ||||
| 34. Round | 13/04/2010 | Werder Bremen II | - | VfB Stuttgart II | 0:3 | |
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| 35. Round | 18/04/2010 | VfB Stuttgart II | - | Kickers Offenbach | 2:0 | ||||
| 22. Round | 21/04/2010 | VfB Stuttgart II | - | Wuppertaler SV | 1:3 | ||||
| 36. Round | 25/04/2010 | FC Ingolstadt 04 | - | VfB Stuttgart II | 1:1 |
Germany