| 2. Round | 29/07/2009 | Rot-Weiß Erfurt | - | Kickers Offenbach | 0:2 | ||||
| 3. Round | 08/08/2009 | Kickers Offenbach | - | VfL Osnabrück | 2:0 | ||||
| 6. Round | 30/08/2009 | Wacker Burghausen | - | Kickers Offenbach | 1:0 | ||||
| 7. Round | 02/09/2009 | Kickers Offenbach | - | Eintracht Braunschweig | 3:0 | ||||
| 9. Round | 11/09/2009 | Jahn Regensburg | - | Kickers Offenbach | 1:1 | ||||
| 10. Round | 19/09/2009 | Kickers Offenbach | - | FC Ingolstadt 04 | 0:1 | ||||
| 11. Round | 26/09/2009 | SpVgg Unterhaching | - | Kickers Offenbach | 1:2 | ||||
| 12. Round | 03/10/2009 | Kickers Offenbach | - | FC Carl Zeiss Jena | 4:0 |  |
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| 14. Round | 24/10/2009 | Kickers Offenbach | - | 1. FC Heidenheim 1846 | 2:1 | ||||
| 16. Round | 07/11/2009 | Kickers Offenbach | - | VfB Stuttgart II | 2:0 | ||||
| 19. Round | 05/12/2009 | Bayern München II | - | Kickers Offenbach | 2:1 | ||||
| 20. Round | 12/12/2009 | Erzgebirge Aue | - | Kickers Offenbach | 4:2 | ||||
| 25. Round | 20/02/2010 | Kickers Offenbach | - | Wacker Burghausen | 3:0 | ||||
| 37. Round | 30/04/2010 | SV Sandhausen | - | Kickers Offenbach | 1:1 | |
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| 38. Round | 08/05/2010 | Kickers Offenbach | - | Bayern München II | 4:1 |  |
Germany