| 1. Round | 15/08/2009 | Bayern München [Youth] | - | 1. FSV Mainz 05 [Youth] | 1:3 |  |
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| 2. Round | 23/08/2009 | 1. FSV Mainz 05 [Youth] | - | SpVgg Greuther Fürth [Youth] | 4:3 | ||||
| 3. Round | 30/08/2009 | SC Freiburg [Youth] | - | 1. FSV Mainz 05 [Youth] | 1:3 | ||||
| 4. Round | 13/09/2009 | 1. FSV Mainz 05 [Youth] | - | 1. FC Nürnberg [Youth] | 1:0 | ||||
| 5. Round | 27/09/2009 | VfB Stuttgart [Youth] | - | 1. FSV Mainz 05 [Youth] | 3:2 | ||||
| 6. Round | 03/10/2009 | 1. FSV Mainz 05 [Youth] | - | SV Darmstadt 98 [Youth] | 3:2 | |
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| 7. Round | 18/10/2009 | Jahn Regensburg [Youth] | - | 1. FSV Mainz 05 [Youth] | 1:2 | ||||
| 8. Round | 31/10/2009 | 1. FSV Mainz 05 [Youth] | - | 1899 Hoffenheim [Youth] | 1:2 | ||||
| 9. Round | 07/11/2009 | Karlsruher SC [Youth] | - | 1. FSV Mainz 05 [Youth] | 3:0 | ||||
| 12. Round | 11/11/2009 | 1. FSV Mainz 05 [Youth] | - | Eintracht Frankfurt [Youth] | 3:2 | ||||
| 10. Round | 14/11/2009 | 1. FSV Mainz 05 [Youth] | - | Waldhof Mannheim [Youth] | 3:0 | ||||
| 11. Round | 21/11/2009 | 1. FC Kaiserslautern [Youth] | - | 1. FSV Mainz 05 [Youth] | 1:2 |
Bosnia-Herzegovina