1st(1st leg) FC Sheriff - Olympiakos Piraeus 0:2 (0:0)
2nd (2nd leg) Olympiakos Piraeus - FC Sheriff 1:0 (0:0)
1st(1st leg): 18/08/2009 19:45 2nd (2nd leg): 26/08/2009 19:45 Winner: Olympiakos Piraeus
1st(1st leg) FC København - APOEL Nikosia 1:0 (0:0)
2nd (2nd leg) APOEL Nikosia - FC København 3:1 (3:1)
1st(1st leg): 18/08/2009 19:45 2nd (2nd leg): 26/08/2009 19:45 Winner: APOEL Nikosia
1st(1st leg) Celtic FC - Arsenal FC 0:2 (0:1)
2nd (2nd leg) Arsenal FC - Celtic FC 3:1 (1:0)
1st(1st leg): 18/08/2009 19:45 2nd (2nd leg): 26/08/2009 19:45 Winner: Arsenal FC
1st(1st leg) FC Timişoara - VfB Stuttgart 0:2 (0:2)
2nd (2nd leg) VfB Stuttgart - FC Timişoara 0:0 (0:0)
1st(1st leg): 18/08/2009 19:45 2nd (2nd leg): 26/08/2009 19:45 Winner: VfB Stuttgart
1st(1st leg) Sporting CP - ACF Fiorentina 2:2 (0:1)
2nd (2nd leg) ACF Fiorentina - Sporting CP 1:1 (0:1)
1st(1st leg): 18/08/2009 19:45 2nd (2nd leg): 26/08/2009 19:45 Winner: ACF Fiorentina
1st(1st leg) RB Salzburg - Maccabi Haifa 1:2 (0:1)
2nd (2nd leg) Maccabi Haifa - RB Salzburg 3:0 (1:0)
1st(1st leg): 19/08/2009 19:45 2nd (2nd leg): 25/08/2009 19:45 Winner: Maccabi Haifa
1st(1st leg) FK Ventspils - FC Zürich 0:3 (0:1)
2nd (2nd leg) FC Zürich - FK Ventspils 2:1 (1:1)
1st(1st leg): 19/08/2009 19:45 2nd (2nd leg): 25/08/2009 19:45 Winner: FC Zürich
1st(1st leg) Levski Sofia - Debreceni VSC 1:2 (0:1)
2nd (2nd leg) Debreceni VSC - Levski Sofia 2:0 (2:0)
1st(1st leg): 19/08/2009 19:45 2nd (2nd leg): 25/08/2009 19:45 Winner: Debreceni VSC
1st(1st leg) Olympique Lyon - RSC Anderlecht 5:1 (4:0)
2nd (2nd leg) RSC Anderlecht - Olympique Lyon 1:3 (0:3)
1st(1st leg): 19/08/2009 19:45 2nd (2nd leg): 25/08/2009 19:45 Winner: Olympique Lyon
1st(1st leg) Panathinaikos - Atlético Madrid 2:3 (0:1)
2nd (2nd leg) Atlético Madrid - Panathinaikos 2:0 (1:0)
1st(1st leg): 19/08/2009 19:45 2nd (2nd leg): 25/08/2009 19:45 Winner: Atlético Madrid