| 1. Round | 05/09/2020 | Rot-Weiss Essen | - | SC Wiedenbrück | 1:1 | ||||
| 5. Round | 23/09/2020 | Rot-Weiss Essen | - | Rot Weiss Ahlen | 3:2 | ||||
| 7. Round | 02/10/2020 | Rot-Weiss Essen | - | Fortuna Düsseldorf II | 2:0 | ||||
| 8. Round | 10/10/2020 | Fortuna Köln | - | Rot-Weiss Essen | 1:1 | ||||
| 9. Round | 17/10/2020 | Rot-Weiss Essen | - | Preußen Münster | 1:0 | ||||
| 14. Round | 07/11/2020 | SV Bergisch Gladbach 09 | - | Rot-Weiss Essen | 0:2 |  |
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| 15. Round | 09/12/2020 | Rot-Weiss Essen | - | SV 19 Straelen | 4:1 |  |
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| 21. Round | 19/12/2020 | Rot-Weiss Essen | - | FC Wegberg-Beeck | 3:2 | ||||
| 23. Round | 23/01/2021 | Rot-Weiss Essen | - | Bonner SC | 2:0 | ||||
| 28. Round | 26/02/2021 | Fortuna Düsseldorf II | - | Rot-Weiss Essen | 3:0 | ||||
| 31. Round | 20/03/2021 | Rot-Weiss Essen | - | Bor. Mönchengladbach II | 4:0 | ||||
| 33. Round | 03/04/2021 | Rot-Weiss Essen | - | FC Schalke 04 II | 5:0 | ||||
| 38. Round | 08/05/2021 | Wuppertaler SV | - | Rot-Weiss Essen | 1:2 | ||||
| 39. Round | 15/05/2021 | Rot-Weiss Essen | - | Alemannia Aachen | 2:1 |
Germany