| 12. Round | 08/11/2008 | SpVgg Greuther Fürth II | - | Karlsruher SC II | 1:2 | ||||
| 13. Round | 15/11/2008 | Karlsruher SC II | - | Waldhof Mannheim | 2:1 | ||||
| 14. Round | 21/11/2008 | Eintracht Frankfurt II | - | Karlsruher SC II | 2:0 | ||||
| 15. Round | 30/11/2008 | Karlsruher SC II | - | SSV Reutlingen | 2:1 | ||||
| 17. Round | 13/12/2008 | Karlsruher SC II | - | TSV Großbardorf | 2:1 |  |
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| 18. Round | 20/12/2008 | Karlsruher SC II | - | SV Darmstadt 98 | 0:2 | ||||
| 20. Round | 03/03/2009 | Karlsruher SC II | - | SpVgg Unterhaching II | 0:4 | |
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| 24. Round | 28/03/2009 | SSV Ulm 1846 | - | Karlsruher SC II | 6:1 | ||||
| 28. Round | 02/05/2009 | SC Freiburg II | - | Karlsruher SC II | 1:0 | ||||
| 32. Round | 23/05/2009 | SSV Reutlingen | - | Karlsruher SC II | 1:3 | ||||
| 19. Round | 26/05/2009 | SV Wehen Wiesbaden II | - | Karlsruher SC II | 1:0 | ||||
| 33. Round | 30/05/2009 | Karlsruher SC II | - | 1. FC Heidenheim 1846 | 0:2 | ||||
| 34. Round | 06/06/2009 | TSV Großbardorf | - | Karlsruher SC II | 1:3 |
Germany