| 20. Round | 03/03/2009 | Karlsruher SC II | - | SpVgg Unterhaching II | 0:4 | ||||
| 21. Round | 07/03/2009 | Viktoria Aschaffenburg | - | Karlsruher SC II | 2:1 | ||||
| 22. Round | 15/03/2009 | 1. FC Nürnberg II | - | Karlsruher SC II | 2:1 |  |
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| 23. Round | 22/03/2009 | Karlsruher SC II | - | SC Pfullendorf | 2:0 | ||||
| 24. Round | 28/03/2009 | SSV Ulm 1846 | - | Karlsruher SC II | 6:1 | ||||
| 16. Round | 07/04/2009 | 1. FC Heidenheim 1846 | - | Karlsruher SC II | 3:0 | ||||
| 25. Round | 11/04/2009 | Karlsruher SC II | - | 1. FC Eintracht Bamberg | 1:4 | ||||
| 26. Round | 17/04/2009 | TSV 1860 München II | - | Karlsruher SC II | 3:0 | ||||
| 28. Round | 02/05/2009 | SC Freiburg II | - | Karlsruher SC II | 1:0 | ||||
| 29. Round | 09/05/2009 | Karlsruher SC II | - | SpVgg Greuther Fürth II | 2:1 | ||||
| 31. Round | 17/05/2009 | Karlsruher SC II | - | Eintracht Frankfurt II | 0:2 | ||||
| 32. Round | 23/05/2009 | SSV Reutlingen | - | Karlsruher SC II | 1:3 |
Germany