| 2. Round | 14/08/2016 | Carolina RailHawks | - | Puerto Rico FC | 2:2 | ||||
| 2. Round | 28/08/2016 | FC Edmonton | - | Puerto Rico FC | 0:0 | ||||
| 3. Round | 04/09/2016 | Puerto Rico FC | - | Miami FC | 0:3 | ||||
| 3. Round | 24/09/2016 | Miami FC | - | Puerto Rico FC | 0:1 | ||||
| 4. Round | 02/10/2016 | Ottawa Fury | - | Puerto Rico FC | 1:2 | ||||
| 4. Round | 30/10/2016 | Puerto Rico FC | - | Carolina RailHawks | 2:1 |
Colombia